HPTLC Fingerprinting of Isoflavonoids and Metabolites Quantification from in vivo and in vitro Developed Parts of Pueraria tuberosa (Willd.) DC

Abstract

Pueraria tuberosa (Willd.) DC, a medicinally important plant, produces different types of isoflavonoids including Genestein. This plant requires conservation. In present study, callus was developed from in vivo tuber and in vitro seedling. Murashige and Skoog medium supplement with combination of BAP (6-Benzylaminopurine) (1.5mg/l) and 2,4-D (1.5mg/l) were found to be more effective. Fresh in vitro shoots produced from nodal explants of in vitro germinated seedling on MS medium supplement with BAP (1mg/l) and NAA (α-napthaleneacetic acid) (1.5mg/l). In vitro regenerated shoots were transferred to rooting media having IBA (indole-3-butyric acid) (1mg/l); and NAA (0.5mg/l) was found more effective. The phytochemical analysis revealed the presence of alkaloids, carbohydrate, glycosides, saponin, phenols, protein, steroid, tannins, flavonoids and quinone in the extracts of in vivo and in vitro plant samples. Quantitative analysis of in vivo and in vitro parts showed that they are good source of primary and secondary metabolites. The HPTLC (High-performance thin-layer chromatography) analysis was carried out on silica gel 60F₂₅₄ HPTLC aluminium sheets with CAMAG Linomat 5 applicator. The plate was developed using Toluene: ethyl acetate (16:4v/v) mobile phase. Isoflavonoids were detected at 254, 366 and 540nm (after derivatization). These developed fingerprinting of in vivo and in vitro parts of P. tuberosa will help to select ideal part for phytochemical extraction.Keywords: HPTLC fingerprinting, Isoflavonoids, micro-propagation, P. tuberosa, metabolites

Cite this Article

Patel Illa, Hakim Masuma, Rathod Dipika. HPTLC Fingerprinting of Isoflavonoids and Metabolites Quantification from in vivo and in vitro Developed Parts of Pueraria tuberosa (Willd.) DC. Research & Reviews: A Journal of Pharmaceutical Science. 2019; 10(3): 34–45p.