A Novel UPLC Method Development and Validation for the Estimation of Paracetamol and Chlormezanone in Dosage Form

Abstract

A simple, rapid, specific, accurate and precise Ultra High-Performance Liquid Chromatographic (HPLC) method was developed for the estimation of paracetamol and chlormezanone in dosage forms. Water Acquity C18 (100×2.1 mm ID) 1.8 µm was the column used. Methanol: Water: TEA (Tri ethyl amine) in the ratio of 50:50:0.5% (v/v/v) was the optimized mobile phase and pH was adjusted to 7.5 by using Ortho Phosphoric Acid. The flow rate was 0.5 ml/min and effluents were monitored at 266 nm. The method was validated for linearity, accuracy, precision, specificity and sensitivity. The retention time of paracetamol and chlormezanone was found to be 2.35 and 4.46 min, respectively. Limit of Detection (LOD) for paracetamol and chlormezanone were found to be 14.34 and 4.13 µg/ml, respectively and Limit of Quantitation (LOQ) for paracetamol and chlormezanone were found to be 43.48 and 12.53 µg/ml, respectively.

Keywords: Chlormezanone, LOD, LOQ, UPLC, Paracetamol

Cite this Article

Teja Sri Adla, Sandhya Mamindla. A Novel UPLC Method Development and Validation for the Estimation of Paracetamol and Chlormezanone in Dosage Form. Research and Reviews: A Journal of Pharmaceutical Science. 2020; 11(1): 1–8p.