A New RP-HPLC Method for Simultaneous Estimation of Atorvastatin and Fenofibrate in Its Bulk and Tablet Dosage Form

Authors

  • S.K. Godasu Associate Professor, Department of Pharmacy, Sri Indu Institute of Pharmacy, Hyderabad, Telangana, India.
  • M. Sowmya Associate Professor, Department of Pharmacy, Sri Indu Institute of Pharmacy, Hyderabad, Telangana, India.
  • D. Varun Professor, Department of Pharmacy, Sri Indu institute of Pharmacy, Hyderabad, Telangana, India.
  • Narmada Valla Keerthi Associate Professor, Department of Pharmacy, Sri Indu Institute of Pharmacy, Hyderabad, Telangana, India.
  • Mohathasim Billah A Professor, Department of Pharmacy, Sri Indu Institute of Pharmacy, Hyderabad, Telangana, India.

Keywords:

Atorvastatin, Fenofibrate, RP-HPLC, simultaneous estimation

Abstract

Objective: A New method was established for simultaneous estimation of Atorvastatin and Fenofibrate by RP-HPLC method. Methods: Chromatographic separations were carried using Phenomenex C18 column (4.6 × 250 mm, 5 µm) column with a mobile phase composition of 0.1 M Ammonium acetate:acetonitrile (60:40) have been delivered at a flow rate of 1ml/min and the detection was carried out using waters HPLC auto sampler, separation module 2695 with PDA detector at wavelength 236 nm. Results: The retention time for Atorvastatin and Fenofibrate were 2.991 and 5.931 minute respectively. The correlation coefficient values in linearity were found to be 0.999 and concentration range 10-30 μg/ml for Atorvastatin and 160-480 µg/ml for Fenofibrate respectively. For accuracy the total recovery was found to be 99.08% and 100% for Atorvastatin and Fenofibrate. Conclusion: The results of study showed that the proposed RP-HPLC method is a simple, accurate, precise, rugged, robust, fast and reproducible, which may be useful for the routine estimation of Atorvastatin and Fenofibrate in pharmaceutical dosage form.

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Published

2022-01-05

Issue

Vol. 8 No. 3 (2021)

Section

Research Article

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