Open Access Open Access  Restricted Access Subscription or Fee Access

A New RP-HPLC Method for Simultaneous Estimation of Atorvastatin and Fenofibrate in Its Bulk and Tablet Dosage Form

S.K. Godasu, M. Sowmya, D. Varun, Narmada Valla Keerthi, Mohathasim Billah A


Objective: A New method was established for simultaneous estimation of Atorvastatin and Fenofibrate by RP-HPLC method. Methods: Chromatographic separations were carried using Phenomenex C18 column (4.6 × 250 mm, 5 µm) column with a mobile phase composition of 0.1 M Ammonium acetate:acetonitrile (60:40) have been delivered at a flow rate of 1ml/min and the detection was carried out using waters HPLC auto sampler, separation module 2695 with PDA detector at wavelength 236 nm. Results: The retention time for Atorvastatin and Fenofibrate were 2.991 and 5.931 minute respectively. The correlation coefficient values in linearity were found to be 0.999 and concentration range 10-30 μg/ml for Atorvastatin and 160-480 µg/ml for Fenofibrate respectively. For accuracy the total recovery was found to be 99.08% and 100% for Atorvastatin and Fenofibrate. Conclusion: The results of study showed that the proposed RP-HPLC method is a simple, accurate, precise, rugged, robust, fast and reproducible, which may be useful for the routine estimation of Atorvastatin and Fenofibrate in pharmaceutical dosage form.


Atorvastatin, Fenofibrate, RP-HPLC, simultaneous estimation

Full Text:



Barquera S, Pedroza-Tobias A, Medina C, et al. Global overview of the epidemiology of atherosclerotic cardiovascular disease. Arch Med Res. 2015; 46(5): 328–338.

Theodosios Filippatos & Haralampos J Milionis. Treatment of hyperlipidaemia with fenofibrate and related fibrates, Expert Opinion on Investigational Drugs. 2008:17(10):1599-1614

R.A. Mhaske, S. Sahasrabudhe, A.A. Mhaske and D. J. Garole. RP_HPLC method for simultataneous determination of atorvastatin calcium, olmesartan medoxomil, candesartan, hydrochlorothiazide and chlorthalidone – Application to Commercially Available Drug Products. IJPSR, 2012; 3(3): 793-801

Nikita N. Patel, Parag R. Patel, Falguni A. Tandel, Charmy S. Kothari, Shailesh A. Shah.Ratio derivative spectrophotometric method for simultaneous estimation of olmesartan medoxomil and atorvastatin calcium in their combined tablet dosage form. International Journal of Pharmacy and Pharmaceutical Sciences 2012; 4(5): 222-226.

Deepak Kumar Jain*, Pratibha Patel, Sanchit Rajawat and H.S. chandel. Development and validation of RPHPLC method for simultaneous estimation of simvastatin, ezetimibe and fenofibrate in ternary mixtures. IJPAC. 2012: 7(2): 139-144.

Jajam Thriveni, R. Rambabu, J.Venkateswara Rao and S. Vidyadhara. Development and validation of RPHPLC method for simultaneous estimation of rosuvastatin calcium and fenofibrate in bulk and pharmaceutical dosage forms. International Journal of Research in Pharmacy and Chemistry 2013; 3(2): 208-212.

Vishnu P. Choudhari and Anna Pratima Nikalje. Simultaneous estimation of atorvastatin, ezetimibe and fenofibrate in pharmaceutical formulation by RP-LC PDA. Pharm Anal Acta 2010: 1(2): 10000111.

M.S. Kondawar, K.G. Kamble, K.H. Maharshi, M.M. Khandare. UV spectrophotometric estimation of ezetimibe and fenofibrate in bulk drug and dosage form using simultaneous equation method. Int.J. ChemTech Res 2011; 3(2): 749-754.

Kadav A, Vora DN. Stability indicating UPLC method for simultaneous determination of atorvastatin, fenofibrate and their degradation products in tablets. J Pharm Biomed Anal. 2008: 48(1): 120-126.

Farnier M, Freeman MW, Macdonell G, Perevozskaya I, Davies MJ, et al. Efficacy and safety of the coadministration of ezetimibe with fenofibrate in patients with mixed hyperlipidaemia. Eur Heart J. 2005: 26(9): 897-905.


  • There are currently no refbacks.

Copyright (c) 2022 Research & Reviews: A Journal of Drug Design & Discovery