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LCMS/MS Analysis of the Ethylacetate Extract of Securidaca longipedunculata Fresen (Polygalaceae) Stem Bark

Abubakar U. S., Danmalam U. H., Ibrahim H., Maiha B. B., Hadiza R. J., Abdullahi M. S., Bashir M.


The present study aimed to identify some of the phytoconstituents of a fraction (fraction DDK-4) obtained from the column chromatography of the ethylacetate extract of Securidaca longipedunculata Fresen (Polygalaceae). Liquid Chromatography-Mass Spectrometry (LCMS/MS) analysis of fraction DDK-4 was conducted on a Waters Synapt G2 quadrupole time-of-flight mass spectrometer (Milford, MA, USA) using positive mode of ionization. The results showed that 1,7-dihydroxy-2,8-dimethoxy xanthone, 3-(1,3-benzodi-oxol-5-yl-4-morpholinyl)-3-(2-hydroxy-4,6-dimethoxyphenyl)-1-propanone, 5,7-dihydroxy-6-methyl-3-(2’,4’-dihydroxybenzyl)-chroman-4-one, trans-O-methyl-grandmarin, dioctylamine, rhodioloside, punicic acid, echimidine, N-[(2R,3S)-2-[[(3,5-dimethyl-4-isoxazolyl)sulfonyl-methylamino]methyl]-5-[(2R)-1-hydroxypropan-2-yl]-3-methyl-6-oxo-3,4-dihydro
-2H-1,5-benzoxazocin-10-yl]-1,3-benzothiazole-2-carboxamide, scilliroside and geranyl hydroquinone were identified as the phytoconstituents present in fraction DDK-4. The presence of these compounds in the stem bark of S. longipedunculata has been reported for the first time. Also, 1,7-dihydroxy-2,8-dimethoxy xanthone could be very useful in the chemotaxonomy of the genus Securidaca and family Polygalaceae. Studies are currently on-going to isolate these compounds in their pure forms in order to elucidate and characterize their structures using different spectroscopic techniques.


Chemotexonamy, Column chromatography, Family, Genus, LCMS/MS, Phytoconstituents

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