In vitro Antioxidant Activity of Calotropis Procera

Abstract

Antioxidant activity of root of Calotropis procera extract was evaluated in a series of in vitro assay involving free radicals. IC₅₀ values were determined. Extracts of I. coccinea exhibited scavenging effect in concentration dependent manner in all in vitro method. IC50 of ethyl acetate extract was 48.43, 75.811 and 60.60 μg/ml for 2,2-diphenylpicrylhydrazyl radical scavenging, hydrogen peroxide scavenging, and nitric oxide scavenging, respectively. Ethyl acetate extract shows good in vitro antioxidant activity compared to other extract.

Keywords: Calotropis procera, DPPH free radical, nitric oxide radical, hydrogen peroxide scavenging