Betazen’ Herbal Formulation, Ameliorates Experimentally Induced Metabolic Disturbances in Insulin Resistant Rats

Abstract

The present study was designed to evaluate anti-diabetic activity of ayurvedic formulation ‘Betazen’ in experimentally induced insulin resistant rats. Wistar rats were being rendered insulin resistant by administration of dexamethasone (2 μg/day s.c), along with high fat-high sugar (HFHS) diet (i.e. vanaspati ghee and fructose enriched) for 42 days. Animals with fasting blood glucose levels (BGL) of more than 150 mg/dl were selected, divided into different groups and administered 2.7 mg/kg pioglitazone, 45 mg/kg, and 90 mg/kg betazen herbal formulation (BHF) respectively, suspended in 1% CMC for 30 days p.o. At the end of the study, animals were sacrificed; blood was collected and used for estimation of various biochemical parameters and HbA_1c. Whereas, glycogen content in the liver and gastrocnemius muscle, glucose uptake in hemi-diaphragm and antioxidant biomarkers in liver were also estimated. Pancreas were subjected for histopathological studies. The results obtained from the present study exhibited a significant dose dependent decrease in elevated fasting BGL, lipids (TC, TG, HDL, LDL and VLDL), HbA_1c and MDA levels. Further, there was a significant (p<0.001) increase in glycogen content, glucose uptake, HDL, and antioxidant biomarkers viz.; GSH, SOD and CAT. These experimental results highlighted the antioxidant, anti-glycation and anti-hyperglycemic potential of BHF on insulin resistance. It further exhibited an improvement in dyslipidemia, insulin sensitivity, and glycogen content. Hence, BHF could be a promising future herbal treatment for insulin resistance type-2 diabetes mellitus.

Keywords: Insulin resistance, dexamethasone, high fat-high sugar, betazen

Cite this Article

Khatib Nayeem A, Caiado Ira CS, Patil Srinivas M et al. ‘Betazen’ Herbal Formulation, Ameliorates Experimentally Induced Metabolic Disturbances in Insulin Resistant Rats. Research & Reviews: A Journal of Pharmacology. 2016; 6(2): 13–23p.