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Preparation, Phytochemical Screening and Pharmacological Evaluation of Poly-herbal Formulation
Abstract
Abstract
The current investigation was endeavored at formulating and evaluating a poly-herbal formulation. The poly-herbal formulation was a combination of 10 herbal ingredients which was phyto-chemically screened for the detection of various phyto-constituents. The formulation was subjected to in vitro Anti-Arthritic and Anthelmintic activity studies. Anti-Arthritic activity was evaluated using various concentrations of the formulation (100 μg/ml, 250 μg/ml, and 500 μg/ml) and Diclofenac sodium (100 μg/ml, 250 μg/ml, and 500 μg/ml), the reference standard. The percentage inhibition of protein denaturation was determined. Anthelmintic activity of the preparation was evaluated on Indian Earthworm in which paralysis time and death time shown by the test preparation (50 mg/ml and 100 mg/ml) was compared with that of the standard Albendazole (50 mg/ml and 100 mg/ml). The outcome of the present study signifies that the preparation showed the presence of various phyto-constituents such as alkaloids, tannins, flavanoids, steroids, glycosides etc. and showed noteworthy anti-arthritic activity and Anthelmintic activity. The higher concentration of PHF was found to possess better anti-arthritic activity and Anthelmintic activity as compared to the formulation at a lower concentration. The Anthelmintic activity and anti-arthritic activity of the prepared PHF was comparable with that of the activity possessed by the standard drug Albendazole and Diclofenac sodium respectively.
Keywords: Albendazole, Anthelmintic, Petrochemicals, Pheretimaposthuma, PHF
Cite this Article
N.L. Gowrishankar, Revathy C., Fathima Shaibana, Haritha C., Janees P.M., Shana Sharin A.P., Sreelakshmi K.P. Preparation, Phytochemical Screening and Pharmacological Evaluation of Poly-herbal Formulation. Research & Reviews: A Journal of Pharmacology. 2019; 9(3): 9–14p.
References
Robert B. Saper et al, Lead, Mercury, and Arsenic in US- and Indian-Manufactured
Ayurvedic Medicines Sold via the Internet MD, MPH2008; 300(8):915-923.doi:
1001/jama.300.8.915.
S Chatterjee, SN Das, Anti-arthritic and anti-inflammatory effect of a poly-herbal drug
(EASE): Its mechanism of action, 1996, Volume: 28, Issue: 2, Page: 116-119.
Asheesh Gupta PhDetal.,A poly‐herbal formulation accelerates normal and impaired diabetic
wound healingVolume16, Issue6 November–December 2008 Pages 784-790
Balsano C, Alisi A. Antioxidant effects of natural bioactive compounds. Curr Pharm
Des.2009; 15: 3063–73.
Canter P. H, Ernst E. Herbal supplement use by persons aged over 50 years in Britain:
Frequently used herbs, concomitant use of herbs, nutritional supplements and prescription
drugs, rate of informing doctors and potential for negative interactions. Drugs
aging.2004;21:597–605
ChandraprakashDwivedi SwarnaliDaspaul, Antidiabetic Herbal Drugs and Polyherbal
Formulation Used For Diabetes, 2013; 2 (3): 44-51.
Selamawit Zenebe, 1 Teka Feyera,1 and Solomon AssefaIn Vitro Anthelmintic Activity of
Crude Extracts of Aerial Parts of Cissusquadrangularis L. and Leaves of Schinusmolle L.
against HaemonchuscontortusVolume 2017 page no;1-6.
Hatem A. Shalaby*,Anthelmintics Resistance,2013 Jan-Mar; 8(1): 18–32
BullockJ.a · RizviS.A.A.b et al.,Rheumatoid Arthritis: A Brief Overview of the treatment,
;27:501–507.
M.chaturvedi,S.Dwivedy,A Dwidevi, Pk Barpet,R-sachan.Formulation and evaluation of
polyherbal anthelmintic preparation-ethanobotanical leaflets 2009(2), 7, 2019.
Zheng W, Wang SY: Antioxidant activity and phenolic compounds in selected herbs. J
Agric Food Chem. 2001, 49 (11): 5165-5170.
Sala A, Recio MD, Giner RM, Manez S, Tournier H, Schinella G, Rios JL: Antiinflammatory and antioxidant properties of Helichrysumitalicum. J Pharm Pharmacol. 2002,
(3): 365-371.
RahmanGul et al., Preliminary phytochemical screening, quantitative analysis of alkaloids,
and antioxidant activity of crude plant extracts from Ephedra intermedia Indigenous to
Balochistan Volume 2017 pg.no : 2-5
Nidal Jaradat et al ,preliminary Phytochemical screening,quantitative estimation of total
flavonoids,total phenols and antioxidant activity of ephedra alata Decne vol
,pg.no.1771-1777
M. Glavas-Dodov, E. Fredro-Kumbaradzi, K. Goracinova, S. Calis, M. Simonoska, and A. A.
Hincal, “5-Fluorouracil in topical liposome gels for anticancer treatment-formulation and
evaluation,” ActaPharmaceutica, vol. 53, no. 4, pp. 241–250, 2003.
Kirtikar K.R, Basu, B.D. Indian medicinal plants. 2006; II: 936-938
Kokate C.K. Practical Pharmacognosy, IV edition. NiraliPrakashan. 1994; 108-109. (1994).
Ajaiyeoba E.O., Onocha P.A., Olorenwaju O.T., Pharm Biol, 2001, 39, 217-220.
Kala Chandra et al.proteection against FCA induced oxidative stress induced DNA damaged
assay model of arthritic potential of Costus speciosus Rhizome extract international journal
of pharmacognosy and phytochemical research 2015,v0l -7,383-389.
Suchita V Ghumre et al assessment of in-vitro anti-inflammatory activity of cynodon
Dactylon and acyclovir showing synergistic effect by albumin denaturation and membrane
stabilization assay,2017.pg no.1-5.
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