Isolation, Phytochemical Investigation and Biological Screening of Roots of Cosmos Sulphureus

Authors

  • Lata Khani Bisht T John College of Pharmacy Bangalore

DOI:

https://doi.org/10.37591/rrjopc.v10i1.1249

Keywords:

Cosmos Sulphureus plant

Abstract

Aims and objectives: The plants are integral part of traditional system of medicine. Cosmos is one such medicinal plant traditionally being used in Brazil and Mexico for treatment of several diseases.

              The present study is aimed to evaluate the properties of Cosmos sulphureus and its preliminary phytochemical screening, extraction, purification and identification of active components.

Materials and Methods:

All roots were handpicked and washed with tap water. Cleaned roots were air dried under shade for 2 to 3 weeks, powdered and subjected to extraction with petroleum ether and methanol. Phytochemical screening was carried out to find the constituents followed by antioxidant activity using DPPH (2, 2-diphenyl-1-picrylhydrazyl) method.

Result and conclusion:

IC50 values for antioxidant potential of petroleum ether and methanol root extracts were found to be 145.5 µg/ml and 75.7 µg/ml respectively. The present work revealed that root part of acetone extract possesses maximum percentage of antioxidant activity. This observation revealed that extracts exhibited great potential antioxidant property. Thus it can be consider as very useful plant to treat and control several diseases.

Key words: Cosmos sulphureus, Extracts, Antioxidants, Anthocyanion, DPPH,

 

Author Biography

  • Lata Khani Bisht, T John College of Pharmacy Bangalore

    ASSOCIATE PROFESSOR,

    PHARMACEUTICAL CHEMISTRY DEPARTMENT,

    T JOHN COLLEGE OF PHARMACY,

    BANGALORE

References

Malaka R, Hema JA, Muthukumarasamy NP, Sambandam A, Subramanian S, Sevanan M. Green synthesis of silver nanoparticles using Cosmos sulphureus and evaluation of their antimicrobial and antioxidant properties. Nano Biomed Eng. 2015;7(4):160-8.

Wink M. Introduction: biochemistry, physiology and ecological functions of secondary metabolites. Biochemistry of plant secondary metabolism. 2010 May 40 (7):1-9.

Shimokoriyama M, Hattori S. Anthochlor pigments of Cosmos sulphureus, Coreopsis lanceolata and C. saxicola. Journal of the American Chemical Society. 1953 Apr;75(8):1900-4.

Moldovan Z, Buleandră M, Oprea E, Mînea Z. Studies on chemical composition and antioxidant activity of Rudbeckia triloba. Journal of Analytical Methods in Chemistry. 2017 Nov 27;2017

Singleton VL, Orthofer R, Lamuela-Raventós RM. [14] Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods in enzymology. 1999 Jan 1;299:152-78.

KolevaII, VanBeek TA, Linssen JPH, de Groot A, Evstatieva LN. Screening of plant extracts for antioxidant activity: a comparative study on three testing methods. Phytochemical Analysis.2002.13 : 8-17.

Lutomski J, Malek B. Pharmacological investigations on raw materials of the genus passiflora. The comparsion of contents of alkaloids in some harman raw materials (author's transl). Plantamedica 1975;27(4):381

Duh PD , Antioxidant Activity of Burdock (ArctiumlappaLinne).Its scavenging effect on free radical and active oxygen, J oil chem soci 1998.75, 455-461.

Nabavi SM, Ebrahimzadeh MA, Nabavi SF, Fazelian M, Eslami B. In vitro antioxidant and free radical scavenging activity of Diospyros lotus and Pyrus boissieriana growing in Iran. Pharmacognosy magazine. 2009 Apr 1;5(18):122.

Downloads

Published

2023-04-08

Issue

Vol. 10 No. 1 (2023)

Section

Research Article

License

Declaration and Copyright Transfer Form

(to be completed by authors)

I/ We, the undersigned author(s) of the submitted manuscript, hereby declare, that the above manuscript which is submitted for publication in the STM Journals(s), is not published already in part or whole (except in the form of abstract) in any journal or magazine for private or public circulation, and, is not under consideration of publication elsewhere.

  • I/We will not withdraw the manuscript after 1 week of submission as I have read the Author Guidelines and will adhere to the guidelines.
  • I/We Author(s ) have niether given nor will give this manuscript elsewhere for publishing after submitting in STM Journal(s).
  • I/ We have read the original version of the manuscript and am/ are responsible for the thought contents embodied in it. The work dealt in the manuscript is my/ our own, and my/ our individual contribution to this work is significant enough to qualify for authorship.
  •  I/We also agree to the authorship of the article in the following order:

Author’s name                                    

 

1. ________________

2. ________________

3. ________________

4. ________________

 

We  Author(s) tick this box and would request you to consider it as our signature as we agree to the terms of this Copyright Notice, which will apply to this submission if and when it is published by this journal.