Antifungal, Antioxidant Activity and Phytochemical Analysis of Extracts from Vitex rivularis (Lamiaceae)

Authors

  • Marguerite Simo University of Yaounde 1
  • Zheng Chen
  • Gaël Siwe
  • Stella Majoumouo
  • Taffou .
  • Maddalena Donati
  • Rufin Toghueo
  • Pierre Jazet
  • Modeste Sameza
  • Fabrice Fekam
  • Guglielmina Froldi

Abstract

The present study was designed to investigate the biological properties of methanolic extracts of leaves and seeds from Vitexrivularis. Leaves and seeds extracts were prepared by maceration in methanol. Phytochemicals analysis was performed by colorimetric method, chemical reaction method and high-performance liquid chromatography (HPLC). The broth microdilution method was used to evaluate the in vitro activity against three strains of Candida albicans and 17 isolates of two yeast species (Candida sp. and Cryptococcus neoformans). The antioxidant activity was evaluated using 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and oxygen radical absorbance capacity (ORAC) assays. The major classes of compounds identified by qualitative phytochemical screening were glycosides, alkaloids and phenols, tannins, flavonoids, anthocyanins and triterpenes. The seeds extract had the highest quantity of phenolic compounds whereas the leaves extract was richerin flavonoids. HPLC analysis revealed the presence of catechin and chlorogenic acid in both the extracts. The minimum inhibitory concentrations (MICs) of methanolic extracts of leaves and seeds from V.rivularis ranged from 3.75 to 15.00 mg/ml. Both the extracts showed scavenging activity with EC50 of 25.31 µg/ml and 45.12 µg/mlfor leaves and seeds extracts, respectively. The results of this study provide an important basis for the use of V. rivularis to control infectious diseases caused by Candida sp. and C.neoformans.

 

Keywords: antifungal, antioxidant, extracts, flavonoids, phenolic compounds

 

Cite this Article

Marguerite Simo, Zheng Chen, Gaël Siwe, Stella Majoumouo, Taffou, Maddalena Donati, Rufin Toghueo, Pierre Jazet, Modeste Sameza, Fabrice Fekam, Guglielmina Froldi. Antifungal, Antioxidant Activity and Phytochemical Analysis of Extracts from Vitex rivularis (Lamiaceae). Research & Reviews: A Journal of Pharmacognosy. 2020; 7(1): 23–48p.

Author Biography

  • Marguerite Simo, University of Yaounde 1
    Departement of Biocheistry, Faculty of Science, University of Yaounde 1

Downloads

Published

2020-05-20

Issue

Vol. 7 No. 1 (2020)

Section

Research Article

License

Declaration and Copyright Transfer Form

(to be completed by authors)

I/ We, the undersigned author(s) of the submitted manuscript, hereby declare, that the above manuscript which is submitted for publication in the STM Journals(s), is not published already in part or whole (except in the form of abstract) in any journal or magazine for private or public circulation, and, is not under consideration of publication elsewhere.

  • I/We will not withdraw the manuscript after 1 week of submission as I have read the Author Guidelines and will adhere to the guidelines.
  • I/We Author(s ) have niether given nor will give this manuscript elsewhere for publishing after submitting in STM Journal(s).
  • I/ We have read the original version of the manuscript and am/ are responsible for the thought contents embodied in it. The work dealt in the manuscript is my/ our own, and my/ our individual contribution to this work is significant enough to qualify for authorship.
  •  I/We also agree to the authorship of the article in the following order:

Author’s name                                    

 

1. ________________

2. ________________

3. ________________

4. ________________

 

We  Author(s) tick this box and would request you to consider it as our signature as we agree to the terms of this Copyright Notice, which will apply to this submission if and when it is published by this journal.