Evaluation of Anti-Diabetic Activity of Ethanolic Leaf Extract of Amorphophallus paeoniifolius by Streptozotocin and Alloxan-Induced Model Using Danio rerio

Abstract

We believe on natural remedy from ancient time. Ayurveda, Siddha, and Unani medicine system gives sufficient importance to Amorphophallus paeoniifolius for its therapeutic efficiency. A. paeoniifolius is commonly known as “elephant foot yam”. The tuber, corms, and leaf parts are having therapeutic importance. At first, the leaves of the plant were collected from the locality of Hooghly district, West Bengal, India in the month of August. After shade drying and size reduction, maceration process was performed using hydroalcoholic solvent (80:20). Phytochemical tests were performed to identify the presence of alkaloids, glycosides, saponin, inulin, proteins and carbohydrates. A large group of Streptozotocin (STZ) and Alloxan-induced diabetic adult zebrafishes (Danio rerio) were taken, weighed and divided into control, test (A. paeoniifolius leaf extract 15, 30 and 50 mg/dl concentration) and standard groups. At 72 h, all group of zebrafishes were weighed and sacrificed to check their final blood glucose level (Accu-Chek: NC Roche). Leaf extract of A. paeoniifolius showed anti-diabetic activity on zebrafish at 15, 30 and 50 mg/dl concentration. Different concentration of test sample (15, 30 and 50 mg/dl) showed significant anti-diabetic activity on zebrafish model where different standard diabetes-induced model (by using STZ and alloxan) were used.

Keywords: alloxan, Amorphophallus paeoniifolius, Danio rerio, streptozotocin (STZ)

Cite this Article

Mukherjee, Bose. Evaluation of Anti-Diabetic Activity of Ethanolic Leaf Extract of Amorphophallus paeoniifolius by Streptozotocin and Alloxan-Induced Model Using Danio rerio. Research & Reviews: A Journal of Pharmacognosy. 2020; 7(3): 18–29p.