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Simultaneous Estimation of Prazosin and Polythiazide in Bulk and Fixed Dose Combination (Tablets) by RP-HPLC

S K Godasu, D. Varun, Narmada Valla Keerthi, Mohathasim Billah A, S.A. Sreenivas


Objective:A New method was established for simultaneous estimation of Prazosin and Polythiazide by RP-HPLC method. Methods:Chromatographic separations were carried using HPLC equipped with Auto Sampler and PDA Detector, Discovery (150 x 46 mm, 5mm) column with a mobile phase composition of Buffer and ACN: taken in the ratio 60:40 (60:40 v/v) have been delivered at a flow rate of 1ml/min and the detection was carried out using waters HPLC auto sampler, separation module 2695 HPLC system with PDA detector at wavelength 270 nm. Results:The retention time for Prazosin and Polythiazide were 2.316 and 3.176 minute respectively. The correlation coefficient values in linearity were found to be 0.999 and concentration range 10-50 μg/ml for Prazosin and 20-100 µg/ml for Polythiazide respectively. For accuracy the total recovery was found to be 100.12% and 100.34% for Prazosin and Polythiazide. LOD and LOQ for Prazosin 0.079 and 0.240 μg/mL, LOD and LOQ for Polythiazide 0.03 and 0.08 μg/mL. Conclusion:The results of study showed that the proposed RP-HPLC method is a simple, accurate, precise, rugged, robust, fast and reproducible, which may be useful for the routine estimation of in Prazosin and Polythiazide bulk and fixed dose combination form.


Prazosin and Polythiazide, RP-HPLC, Simultaneous estimation

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