Physico-Chemical, Phyto-Chemical and HPTLC Analysis of Root of Melia azedarach Linn

Abstract

Mahanimba, botanically known as Melia azedarach L. is an important medicinal plant of Ayurveda. Different parts of Mahanimba are widely used to treat many ailments. Its root is used in Gridhrasi (sciatica) and also used as an ingredient of many Ayurvedic formulations. Present study was carried out to evaluate physicochemical, phyto-chemical and HPTLC analysis of root of M. azedarach Linn. following standard guidelines. Results of physicochemical study revealed that, root powder has loss on drying 3.71% w/w, total ash content 9.45% w/w, water soluble extractive 15.56% w/w, alcohol soluble extractive 6.00% w/w, and pH (5% aqua solution v/w) 5.5. Tannins, steroid, glycosides were important phyto-constituents of root extract. HPTLC study showed six spots at 254 nm i.e. 0.05, 0.12, 0.25, 0.68, 0.83 and 0.93; and five spots in 366 nm i.e. 0.05, 0.12, 0.20, 0.77 and 0.83. The observations made in this study may help to develop the standards of qualitative and quantitative parameters with regard to identification, quality and purity of Melia azedarach root.

Keywords: Mahanimba, Melia azedarach, physicochemical, phyto-chemical, HPTLC study

Cite this Article

Vekariya Shweta R, Krushnkumar Taviad, Nishteswar Kara et al. Physico-Chemical, Phyto-Chemical and HPTLC Analysis of Root of Melia azedarach Linn. Research and Reviews: A Journal of Pharmaceutical Science. 2016; 7(2): 1–5p.