HPTLC: An Important Tool for Estimation of Phytoconstituents

Abstract

HPTLC (High-performance thin layer chromatography) technique is widely employed in pharmaceutical industry for the process development, identification and estimation of adulterants in herbal formulations and helps in estimation and identification of pesticide content, mycotoxins and in quality control of nutraceuticals. For the herbal extracts and especially for the botanical drugs, the issue of stability is of exceptional importance. Standardization is required for the development of an accurate biological activity and for maintaining the quality of an herbal formulation. HPTLC is a simple technique and has many advantages over TLC as it is more sensitive techniques which can run more samples in a shorter period of time. The material needed and the methodology used in handling the machine is described in detail. The quality assurance of herbal formulation is an initial step for the herbal drug industry and other drug development organization for meeting the quality standards. It is mainly employed in estimation of the compounds with low or moderate polarities and particular suitable tool for stability testing for herbal medicinal products and for identification of the phytoconstituents available in herbs.

Keywords: HPTLC, Phytoconstituents, Stability, Biological activity, Herbal formulations

Cite this Article

Neeraj Choudhary, HPTLC: An Important Tool for Estimation of Phytoconstituents, Research and Reviews: A Journal of Pharmaceutical Science. 2016; 7(1): 28–33p.