Development and Validation of Liquid Chromatography-Tandem Mass Spectrometric Method for Assay of Zidovudine in Human UrineDevelopment and Validation of Liquid Chromatography-Tandem Mass Spectrometric Method for Assay of Zidovudine in Human Urine

Abstract

A sensitive and selective liquid chromatographic-tandem mass spectrometric (LC-MS-MS) method was developed to determine zidovudine (ZDV) in human urine. After solid-phase extraction with SPE cartridge, the urine sample was analyzed on a C₁₈ column (Symmetry 3.5 µm; 75×4.6 mm i.d) interfaced with a triple quadrupole tandem mass spectrometer. Positive electrospray ionization was employed as the ionization source. The mobile phase consisted of water-acetonitrile (20:80, v/v).The method was linear over a concentration range of 10–1000 ng mL^-1. The lower limit of quantitation was 10 ng mL^-1. The intra-day and inter-day relative standard deviation across three validation runs over the entire concentration range was <9.5%. The accuracy determined at three concentrations (150, 350 and 750 ng mL^-1 ZDV) was within ±0.73% in terms of relative errors.

Keywords: Zidovudine, quantification, liquid chromatography-tandem mass spectrometry, human urine, determination