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Bergenin: Review of The Methods of Determination and Quantification
Abstract
Studies have shown that Bergenin has various pharmacological functions, such as Anti-lithiatic, Anti-oxidant, anti-inflammatory, Anti-bacterial, Anti-viral, Anti-cancer, Anti-cholinesterase, Anti-diabetic, Hypolipidemic, Anti-tussive, Hepatoprotective and Immunomodulatory. Bergenin is found in the Saxifragaceae, Euphorbiaceae, Myrsinaceae, Dipterocarpaceae, and Fabaceae genera and a range of beneficial effects on human health. The number of bioactive compounds in various bergenia species must be determined in order to achieve the best quality raw material for herbal preparations. As a result, developing analytical methods to investigate the pharmacokinetics and activity mechanisms of berberine alkaloids, as well as a more systematic analysis of berberine alkaloids, is worthwhile. Thin-layer chromatography (TLC), high-performance thin layer chromatography (HPTLC), high-performance liquid chromatography (HPLC), mass spectroscopy (MS), and other analytical techniques are used to identify Bergenin. Since plant extracts have complex structures, isolating and identifying active compounds is a complicated, time-consuming, and costly. This present review summarizes analytical method for identification and quantification of Bergenin.
Keywords
References
Paterson I, Anderson EA. The renaissance of natural products as drug candidates. Science (80-). 2005;310(5747):451–3.
Taneyama M, Yoshida S. Incorporation of 14C-glucose into bergenin and arbutin in Saxifraga stolonifera. Bot Mag Tokyo. 1979;92(1):69–73.
Hay JE, Haynes LJ. Bergenin, a C-glycopyranosyl derivative of 4-O-methylgallic acid. J Chem Soc. 1958;2231–8.
Li X, Zou R, Niu Y, Shao T, et al. Voltammetric determination of bergenin with graphene modified glassy carbon electrode. Int J Electrochem Sci. 2018;13(2):1976–84.
Dharmender R, Madhavi T, Reena A, et al. Simultaneous Quantification of Bergenin, (+)-Catechin, Gallicin and Gallic acid; and Quantification of β-Sitosterol using HPTLC from Bergenia ciliata (Haw.) Sternb. Forma ligulata Yeo (Pasanbheda). Pharm Anal Acta. 2010;01(01).
Nunomura RCS, Oliveira VG, Da Silva SL, et al. Characterization of bergenin in endopleura uchi bark and its anti-inflammatory activity. J Braz Chem Soc. 2009;20(6):1060–4.
Bharate SB, Kumar V, Bharate SS, et al. Discovery and preclinical development of IIIM-160, a Bergenia ciliata-based anti-inflammatory and anti-arthritic botanical drug candidate. J Integr Med. 2019;17(3):192–204. : https://doi.org/10.1016/j.joim.2019.03.001
De Oliveira CM, Nonato FR, De Lima FO, Couto RD, et al. Antinociceptive properties of bergenin. J Nat Prod. 2011;74(10):2062–8.
Zhang C, Zhao B, Zhang C, et al. Mechanisms of bergenin treatment on chronic bronchitis analyzed by liquid chromatography-tandem mass spectrometry based on metabolomics. Biomed Pharmacother. 2019;109(September 2018]:2270–7. : https://doi.org/10.1016/j.biopha.2018.11.119
Boros B, Jakabová S, Madarász T, et al. Validated HPLC method for simultaneous quantitation of bergenin, arbutin, and gallic acid in leaves of different Bergenia species. Chromatographia. 2014;77(17–18):1129–35.
Ruby KM, Chauhan R, Sharma S, Dwivedi J. Polypharmacological activities of bergenia species. Int J Pharm Sci Rev Res. 2012;13(1):100–10.
Chinaka CN, Garuba AS, Ahmadu AA. Chemical Constituents from the Stem Bark of Pentaclethra. Nig J Pharm Res. 2017;13(1):37–44.
Shi X, Xu M, Luo K, et al. Anticancer activity of bergenin against cervical cancer cells involves apoptosis, cell cycle arrest, inhibition of cell migration and the STAT3 signalling pathway. Exp Ther Med. 2019;3525–9.
Chen Z, Liu YM, Yang S, et al. Studies on the chemical constituents and anticancer activity of Saxifraga stolonifera (L) Meeb. Bioorganic Med Chem. 2008;16(3):1337–44.
Roselli M, Lentini G, Habtemariam S. Phytochemical, antioxidant and anti-α-glucosidase activity evaluations of Bergenia cordifolia. Phyther Res. 2012;26(6):908–14.
He S, Li F, Zhou D, et al. Formulation and evaluation of novel coated floating tablets of bergenin and cetirizine dihydrochloride for gastric delivery. Drug Dev Ind Pharm. 2012;38(10):1280–8.
Kumar R, Patel DK, Prasad SK, et al. Type 2 antidiabetic activity of bergenin from the roots of Caesalpinia digyna Rottler. Fitoterapia. 2012;83(2):395–401. Available from: http://dx.doi.org/10.1016/j.fitote.2011.12.008
Aggarwal D, Kaushal R, Kaur T, et al. The most potent antilithiatic agent ameliorating renal dysfunction and oxidative stress from Bergenia ligulata rhizome. Vol. 158, Journal of Ethnopharmacology. 2014. p. 85–93.
PAN RH, HE HM, DAI Y, et al. Comparative pharmacokinetics of bergenin, a main active constituent of Saxifraga stolonifera Curt., in normal and hepatic injury rats after oral administration. Chin J Nat Med. 2016;14(10):776–82.http://dx.doi.org/10.1016/S1875-5364(16)30092-9
Ye YP, Sun HX, Pan YJ. Bergenin monohydrate from the rhizomae of Astilbe chinensis. Acta Crystallogr Sect C Cryst Struct Commun. 2004;60(6):397–8.
De Dieu Dongmo J, Akak CM, Tala MF, et al. Longiflorol, a bergenin α-d-apioside from the stem bark of Diospyros longiflora, and its antioxidant activity. Vol. 73, Zeitschrift fur Naturforschung - Section B Journal of Chemical Sciences. 2018. p. 539–43.
de Oliveira GAL, da Silva Oliveira GL, Nicolau LAD, et al. Bergenin from Peltophorum dubium: Isolation, Characterization, and Antioxidant Activities in Non-Biological Systems and Erythrocytes. Med Chem (Los Angeles). 2017;13(6):592–603.
Tacon LA, Freitas LAP. Box-Behnken design to study the bergenin content and antioxidant activity of Endopleura uchi bark extracts obtained by dynamic maceration. Brazilian J Pharmacogn. 2013;23(1):65–71.
Srivastava N, Singh BN, Srivastava A, et al. Evaluation of Phenolic Content Recoveries in Hydrolyzed Extracts of Bergenia ciliata Using RP-HPLC, GC-MS after Silylation, and Validation through Antioxidant Potential. J Liq Chromatogr Relat Technol. 2015;38(19):1722–30.
Rastogi S, Rawat A. A comprehensive review on bergenin, a potential hepatoprotective and antioxidative phytoconstituent. Herba Pol. 2008;54(2):66–79.
De Abreu HA, Aparecida Dos S. Lago I, Souza GP, et al. Antioxidant activity of [+]-bergenin-a phytoconstituent isolated from the bark of Sacoglottis uchi Huber [Humireaceae]. Org Biomol Chem. 2008;6[15]:2713–8.
Singh M, Pandey N, Agnihotri V, et al. Antioxidant, antimicrobial activity and bioactive compounds of Bergenia ciliata Sternb.: A valuable medicinal herb of Sikkim Himalaya. J Tradit Complement Med. 2017;7[2]:152–7.http://dx.doi.org/10.1016/j.jtcme.2016.04.002
Vaishali JS, Piyush MP. Bergenin - An Active Constituent Of Rivea Ornata Roxb. And Its Antioxidant Property. Int J Pharmacogn. 2017;4[9]:309–19.
YANG Zhu, HUANG Jing-hui, WANG Nai-lin YX. New bergenin derivatives and the antioxidant activity from Ardisia gigantifolia Stapf. J Shenyang Pharm Univ. 2008;1.
Yun J, Lee Y, Yun K, Oh S. Bergenin decreases the morphine-induced physical dependence via antioxidative activity in mice. Arch Pharm Res. 2015;38[6]:1248–54.
Nazir N, Koul S, Qurishi MA, et al. Evaluation of antioxidant and antimicrobial activities of Bergenin and its derivatives obtained by chemoenzymatic synthesis. Eur J Med Chem. 2011;46[6]:2415–20.http://dx.doi.org/10.1016/j.ejmech.2011.03.025
Khan H, Amin H, Ullah A, Saba S, et al. Antioxidant and Antiplasmodial Activities of Bergenin and 11- O -Galloylbergenin Isolated from Mallotus philippensis. Oxid Med Cell Longev. 2016;2016.
Dong G, Zhou Y, Song X. In vitro inhibitory effects of bergenin on human liver cytochrome p450 enzymes. Pharm Biol. 2018;56[1]:620–5.https://doi.org/10.1080/13880209.2018.1525413
Nazir N, Koul S, Qurishi MA, et al. Immunomodulatory effect of bergenin and norbergenin against adjuvant-induced arthritis-A flow cytometric study. J Ethnopharmacol. 2007;112[2]:401–5.
Villarreal CF, Santos DS, Lauria PSS, et al. Bergenin Reduces Experimental Painful Diabetic Neuropathy by Restoring Redox and Immune Homeostasis in the Nervous System. Int J Mol Sci. 2020;21:4850.doi:10.3390/ijms21144850
Haribabu K, Ajitha M, Ramesh B, et al. Quantification of bergenin from Mallotus philippinensis by HPTLC-MS and study on different extraction methods. J Planar Chromatogr - Mod TLC. 2012;25[5]:445–9.
Liang C, Pei S, Ju W, et al. Synthesis and in vitro and in vivo antitumour activity study of 11-hydroxyl esterified bergenin/cinnamic acid hybrids. Eur J Med Chem. 2017;133:319–28.http://dx.doi.org/10.1016/j.ejmech.2017.03.053
WD Chen and MH Nie. HPLC DETERMINATION OF BERGENIN IN ASTILBE CHINENSIS AND BERGENIA PURPURASCENS. Acta Pharm Sin. 1988;8.
Han LK, Ninomiya H, Taniguchi M, et al. Norepinephrine-augmenting lipolytic effectors from Astilbe thunbergii rhizomes. J Nat Prod. 1998;61[8]:1006–11.
Guan S, Qin X, Zhou Z, et al. Investigation of the mechanisms of improved oral bioavailability of bergenin using bergenin-phospholipid complex. Drug Dev Ind Pharm. 2014;40[2]:163–71.
Pozharitskaya ON, Ivanova SA, Shikov AN, et al. Separation and evaluation of free radical-scavenging activity of phenol components of green, brown, and black leaves of Bergenia crassifolia by using HPTLC-DPPH method. J Sep Sci. 2007;30[15]:2447–51.
Chernetsova ES, Crawford EA, Shikov AN, et al. ID-CUBE direct analysis in real time high-resolution mass spectrometry and its capabilities in the identification of phenolic components from the green leaves of Bergenia crassifolia L. Vol. 26, Rapid Communications in Mass Spectrometry. 2012. p. 1329–37.
Dhalwal K, Shinde VM, Biradar YS, et al. Simultaneous quantification of bergenin, catechin, and gallic acid from Bergenia ciliata and Bergenia ligulata by using thin-layer chromatography. J Food Compos Anal. 2008;21[6]:496–500.
Shrestha UK, Pant B. Production of bergenin, an active chemical constituent in the callus of Bergenia ciliata [Haw.] Sternb. Bot Orient J Plant Sci. 2012;8:40–4.
Bhandari L, Bista BB, Khanal C. Phytochemical, Microscopic and Standardization of Bergenia ciliata for Authentication. J Plant Resour. 2019;17[1]:118–24.
Chauhan SK, Singh B, Agrawal S. Simultaneous determination of bergenin and gallic acid in Bergenia ligulata wall by high-performance thin-layer chromatography. J AOAC Int. 2000;83[6]:1480–3.
Pushpalatha H, Pramod K, Devanathan R, et al. Use of bergenin as an analytical marker for standardization of the polyherbal formulation containing Saxifraga ligulata. Pharmacogn Mag. 2015;11[42]:60.
Li BH, Wu JD, Li XL. LC-MS/MS determination and pharmacokinetic study of bergenin, the main bioactive component of Bergenia purpurascens after oral administration in rats. J Pharm Anal. 2013;3[4]:229–34.http://dx.doi.org/10.1016/j.jpha.2013.01.005
Ying C. Chemical constituents from Bergenia scopulosa [ Ⅰ ]. Chinese Tradit Pat Med. 2011;9.
Yong-hui F. Determination of Bergenin in the RHIZOMA BERGENIAE SCOPULOSAE by HPLC. J Anhui Agric Sci. 2011;26.
LUO Ding-qiang, ZHANG Guo-yue LT. Determination of bergenin in different parts of rodgersia aesculifolia batal by HPLC. Anhui Med Pharm J. 2008;12.
REN Jing, LI Duo-wei, CHEN Li-hong, et al. REVERSED-PHASE HPLC DETERMINATION OF BERGENIN IN RODGERIS AESCULIFOLIA BATAL. Phys Test Chem Anal Part B Chem Anal. 2005;5.
Deng J, Xiao X, Tong X, Li G. Preparation of bergenin from Ardisia crenata sims and Rodgersia sambucifolia hemsl based on microwave-assisted extraction/high-speed counter-current chromatography. Sep Purif Technol. 2010;74[2]:155–9.http://dx.doi.org/10.1016/j.seppur.2010.05.018
Wen-wen C, Qin-wan H, Yan LAN, et al. Content determination of bergenin in ethnodrug Rodgersia sambucifolia Hemsl. by HPLC. West China J Pharm Sci. 2007;2.
Zhou M, Chen HG, Xian C, et al. HPLC fingerprint of ethyl acetate extraction of Saxifraga stolonifera. Zhongguo Zhongyao Zazhi. 2013;38[7]:1026–9.
Zhang M, Liu D, Zhang YQ. Rapid analysis of the bioactive components in Saxifraga stolonifera, an edible and medicinal herb with anti-tumor effects, by HPLC-DAD, ESI/MSn. J Appl Bot Food Qual. 2019;92:123–9.
Wijayabandara MDJ, Choudhary MI, et al. Isolation of bergenin from the leaves of Flueggea leucopyrus willd [katupila] – a novel method of obtaining bergenin. Pharm J Sri Lanka. 2015;5[0]:10.
Siddiqui NA, Alam P, Al-Rehaily AJ, et al. Simultaneous quantification of biomarkers bergenin and menisdaurin in the methanol extract of aerial parts of flueggea virosa by validated HPTLC densitometric method. J Chromatogr Sci. 2015;53[5]:824–9.
Jung JC, Lim E, Kim SH, Kim NS, et al. Practical Synthesis and Biological Evaluation of Bergenin Analogs. Chem Biol Drug Des. 2011;78[4]:725–9.
P. Atchuta Ramaiah et. al. Isolation and Characterisation of Bergenin Derivatives from Macaranga peltata. J Chem Soc [Perkin 1]. 1979;2313–6.
Sriset Y, Chatuphonprasert W, Jarukamjorn K. Quantitative Determination of Bergenin in Mallotus repandus [Willd.] Muell. Arg. Stem Extract by Reverse Phase-High Performance Liquid Chromatography. Isan J Pharm Sci. 2018;1[14]:67–74.
Magaji MG, Musa AM, Abdullahi MI, et al. Isolation of bergenin from the root bark of Securinega virosa and evaluation of its potential sleep promoting effect. Avicenna J phytomedicine. 2015;5[6]:587–58796.
Song H, Wang J, Zhang R, et al. In vivo metabolism study of bergenin in rats by HPLC-QTOF mass spectrometry. Vol. 27, Biomedical Chromatography. 2013. p. 1398–405.
Singh R, Kumar V, Bharate SS, et al. Synthesis, pH dependent, plasma and enzymatic stability of bergenin prodrugs for potential use against rheumatoid arthritis. Bioorganic Med Chem. 2017;25[20]:5513–21.http://dx.doi.org/10.1016/j.bmc.2017.08.011
XiangDong C, GuangDa Z, FaJin L, et al. Simultaneous determination of bergenin and quercitroside in Ardisiae Japonicae Herba Dispensing Granules by HPLC. J Guangdong Pharm Univ. 2012;28[5]:522–5.http://branch.gdpu.edu.cn/xuebao/
Wei XH, Yang SJ, Liang N, et al. Chemical constituents of Caesalpinia decapetala [Roth] alston. Molecules. 2013;18[1]:1325–36.
R. Srinivasan, T. Somesh, M.J.N. Chandrasekar, et al. Determination Of Bergenin In Caesalpinia Digyna By HPTLC. Prof Eng. 2007;20[21]:20–1.
R. Srinivasan, R. Gopinath, M. J. Nanjan BS. Determination Of Bergenin In Caesalpinia Digyna Rottler By RP-HPLC Method. Anal Chem. 2006;2[1]:6–9.
Subramanian R, Subbramaniyan P, Raj V. Isolation of bergenin from Peltophorum pterocarpum flowers and its bioactivity. Beni-Suef Univ J Basic Appl Sci. 2015;4[3]:256–61.http://dx.doi.org/10.1016/j.bjbas.2015.06.002
Elufioye T, Obuotor E, Agbedahunsi J, et al. Isolation and Characterizations of Bergenin from Peltophorum pterocarpum Leaves and Its Cholinesterase Inhibitory Activities. European J Med Plants. 2016;11[2]:1–7.
Mukherjee H, Ojha D, Bharitkar YP, et al. Evaluation of the wound healing activity of Shorea robusta, an Indian ethnomedicine, and its isolated constituent[s] in topical formulation. J Ethnopharmacol. 2013;149[1]:335–43.http://dx.doi.org/10.1016/j.jep.2013.06.045
Nasser JA, Yaacob WA, Din LB, et al. Isolation of atranorin, bergenin and goniothalamin from Hopea sangal. ARPN J Eng Appl Sci. 2009;4[1]:92–5.
Anaka TT, To TI, Do YI, et al. Hopeafuran and a C -Glucosyl Resveratrol Isolated from Stem Wood of Hopea utilis. 2001;49[June]:785–7.
Wibowo A, Ahmat N, Hamzah AS, et al. Resveratrol oligomers from the stem bark of Dryobalanops aromatica. Biochem Syst Ecol. 2012;40:62–4.http://dx.doi.org/10.1016/j.bse.2011.09.013
Ito T, Tanaka T, Iinuma M, et al. Two New Resveratrol [=5-[[1E]-2-[4-Hydroxyphenyl]ethenyl]-benzene-1,3-diol] Tetramers with a Tetrahydrofuran Ring from Dipterocarpus grandiflorus. Helv Chim Acta. 2004;87[2]:479–95.
Caldas CS, De Simone CA, Pereira MA, et al. Bergenin monohydrate, a constituent of Hurmiria balsamifera, at 120 K. Vol. 58, Acta Crystallographica Section E Structure Reports Online. 2002. p. o609–11.
Maduka HCC, Okoye ZSC, Eje A. The influence of Sacoglottis gabonensis stem bark extract and its isolate bergenin, Nigerian alcoholic beverage additives, on the metabolic and haematological side effects of 2,4-dinitrophenyl hydrazine-induced tissue damage. Vascul Pharmacol. 2003;39[6]:317–24.
Alves CQ, David JM, David JP, et al. Flavonoids and other bioactive phenolics isolated from cenostigma macrophyllum [Leguminosae]. Quim Nova. 2012;35[6]:1137–40.
Rajbhandari M, Lalk M, Mentel R, et al. Antiviral activity and constituents of the Nepalese medicinal plant Astilbe rivularis. Rec Nat Prod. 2011;5[2]:138–42.
Su Y, Xu JJ, Bi JL, et al. Chemical constituents of Arisaema franchetianum tubers. J Asian Nat Prod Res. 2013 Jan 1;15[1]:71–7.https://www.tandfonline.com/doi/full/10.1080/10286020.2012.723202
Seo JH, Kim JE, Shim JH, et al. HPLC analysis, Optimization of extraction conditions and biological evaluation of corylopsis coreana uyeki flos. Molecules. 2016;21[1]:1–13.
Li C, Chen X, Fang D. A new bergenin derivative from Corylopsis willmottiae. 2011;47[2]:180–1.
CD S, R S, S K. Isolation, Characterization and Quantification of Bergenin From Syzygium Cumini Stem Bark. Int Res J Pharm. 2015;6[2]:108–10.
Tiwari B. K. KRL. Studies on Viburnum nervosum Hook: Chemistry and Spectroscopy of Bergenin and its Derivatives. East Cent African J Pharm Sci. 2009;12:23–5.
Dinesh Kumar, Mohini Sharma, Ashima Sorout, et al. Woodfordia fruticosa Kurz.: A Review on its Botany, Chemistry and Biological activities. J Pharmacogn Phytochem. 2016;5[3]:293–8.
Zamarrud, Ali I, Hussain H, et al. Two new antioxidant bergenin derivatives from the stem of Rivea hypocrateriformis. Fitoterapia. 2011;82[4]:722–5.http://dx.doi.org/10.1016/j.fitote.2011.03.002
Zhao J, Zeng LH, Li X, Dong XP, et al. Brachystemols A-C, three new furan derivatives from Brachystemma calycinum. J Asian Nat Prod Res. 2011;13[10]:915–9.
Ahmed F, Urooj A. Cardioprotective activity of standardized extract of Ficus racemosa stem bark against doxorubicin-induced toxicity. Pharm Biol. 2012;50[4]:468–73.
Zhang Y, Zhang Z, Wang X, Liu H. Determination of Bergenin in Pulmonaria officinalis by High Performance Capillary Electrophoresis. Adv Eng Res. 2018;162[3]:57–61.
Chauhan R, Ruby KM, Dwivedi J. Secondary metabolites found in Bergenia species: A compendious review. Int J Pharm Pharm Sci. 2013;5[1]:9–16.
Patel DK, Patel K, Kumar R, et al. Pharmacological and analytical aspects of bergenin: A concise report. Asian Pacific J Trop Dis. 2012;2[2]:163–7.http://dx.doi.org/10.1016/S2222-1808[12]60037-1
Singh DP, Srivastava SK, Govindarajan R, et al. High-performance liquid chromatographic determination of bergenin in different Bergenia species. Acta Chromatogr. 2007;[19]:246–52.
CHENG Ben, LI Yun-xiang, QUAN Qiu-mei, et al. Comparison of Bergenin Contents of Bergenia emeiensis in Different Altitude in E mei. J China West Norm Univ. 2011;4.
Qin X, Yuan F, Zhou D, Huang Y. Oral characteristics of bergenin and the effect of absorption enhancers in situ, in vitro and in vivo. Arzneimittel-Forschung/Drug Res. 2010;60[4]:198–204.
Yi-kun C, Jin-qing LU, Hong-bing LUO, et al. HPLC Determination of Praeruptorin A, Bergenin and Quercetin in Purpose-made Cigarette and its Smoke. Tob Sci Technol. 2007;1.
Song L ke, Zhou W min. Content determination of bergenin in Ardisia pusilla by HPLC. Zhong Yao Cai. 2007;30[4]:422–3.
Xiu-ping MA. Determination of Bergenin in Ardisia Crenata Sims. By HPLC. Chinese J Ethnomedicine Ethnopharmacy. 2004;6.
Shi Y-B, Yan-Ping Shi and Qing-Gang. Determination and pharmacokinetic study of bergenin in rat plasma by RP-HPLC method. Biomed Chromatogr. 2006;[20]:1065–70.
Srivastava N, Srivastava A, Srivastava S, et al. Simultaneous Quantification of Bergenin, Epicatechin, [+]-Catechin, and Gallicin in Bergenia ciliata Using High Performance Liquid Chromatography. J Liq Chromatogr Relat Technol. 2015;38[12]:1207–12.
Chen Y, Du K, Li J, et al. A Green and Efficient Method for the Preconcentration and Determination of Gallic Acid, Bergenin, Quercitrin, and Embelin from Ardisia japonica Using Nononic Surfactant Genapol X-080 as the Extraction Solvent. Int J Anal Chem. 2018;
Wang J, Wang BJ, Wei CM, et al. Determination of bergenin in human plasma after oral administration by HPLC-MS/MS method and its pharmacokinetic study. Vol. 23, Biomedical Chromatography. 2009. p. 199–203.
Yu W, Wang Y, Zhang Y, et al. Quantitation of bergenin in human plasma by liquid chromatography/tandem mass spectrometry. J Chromatogr B Anal Technol Biomed Life Sci. 2008;11[28].
Song H, Wang J, Zhang R, et al. Identification and characterization of human UDP-glucuronosyltransferases responsible for the in vitro glucuronidation of bergenin. Biomed Chromatogr. 2014;28[3]:348–53.
Chen J, Zhang J, Zhuang Q, et al. Electrochemical study of bergenin on a poly[4-[2-pyridylazo]-resorcinol] modified glassy carbon electrode and its determination in tablets and urine. Talanta. 2007;72[5]:1805–10.
Zhang L, Zhang W, Chen G. Determination of arbutin and bergenin in Bergeniae Rhizoma by capillary electrophoresis with a carbon nanotube-epoxy composite electrode. J Pharm Biomed Anal. 2015;115:323 9.http://dx.doi.org/10.1016/j.jpba.2015.07.044
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