Analytical Method Development and Validation for Simultaneous Estimation of Linagliptin and Empagliflozin by using RP-HPLC–PDA

Authors

  • T. Vinod Kumar Student of M. pharm at school of Pharmaceutical Sciences and Technologies, Jawaharlal Nehru Technological University, Kakinada, Andhra Pradesh, India

Keywords:

Method Development, Validation, Linagliptin, Empagliflozin, RP-HPLC

Abstract

Present my research work done for the measurement and quantification of Linagliptin and Empagliflozin, a rapid, sensitive, and Reliable RP-HPLC technique using a Waters HPLC System with PDA detection was designed and validated. Chromatography was carried out on an Thermo-ODS C18 (150 ×x 4.6 mm, 5) column with a Flow rate of 1.0 ml/min and an effluent of 223 nm using filtered and mixed Degassed potassium dihydrogen phosphate and Methanol: (70:30) as a mobile phase. 

References

P.D. Sethi, HPLC, Pharmaceutical Formulations Quantitative Analysis, 1st edition, CBS publishers and distributors, New Delhi, 2001: 3-39, 116-120.

G.D. Christen, Analytical Chemistry, 6th Edition, John Wiley & Sons, 2003: 1-15.

D.A. Skoog, Instrumental Analytical Concepts, 5th edition, Eastern Press, Banglore, 2004: 1-2, 674-688, 695-698.

B.K. Sharma, Chemical Analytical Instrumental Methods, Introduction to Analytical Chemistry, Meerut: Goel Publishing House, 2000: 1-4, 200-2003

Sharma, B.K., Chemical Analytical Instrumental Methods, Introduction to Analytical Chemistry, Goel Publishing House, Meerut, 21st Edition, 2002: 3-5, 10.

Willard H.H., Instrumental Research Techniques, New Delhi: CBS editors & distributors, 1986: 1-53, 513-522, 530-535.

National Institutes of Health. Linagliptin. Available online at: https://pubchem.ncbi.nlm.nih.gov/compound/Linagliptin

National Institutes of Health. Empagliflozin. Available online at: https://pubchem.ncbi.nlm.nih.gov/compound/Empagliflozin

Staut T, High-Efficiency Liquid Chromatography. Practical Analytics Hnadbook, 1st Edition, New York: Marcel Dekker, Inc; 2005: 117:87-90.

Ohnnesian L, Streeter AJ. Practical Analytics Hnadbook, 1st Edition, New York: Marcel Dekker, Inc; 2005: 117:87-90.

Downloads

Published

2022-01-05

Issue

Vol. 8 No. 3 (2021)

Section

Research Article

License

Declaration and Copyright Transfer Form

(to be completed by authors)

I/ We, the undersigned author(s) of the submitted manuscript, hereby declare, that the above manuscript which is submitted for publication in the STM Journals(s), is not published already in part or whole (except in the form of abstract) in any journal or magazine for private or public circulation, and, is not under consideration of publication elsewhere.

  • I/We will not withdraw the manuscript after 1 week of submission as I have read the Author Guidelines and will adhere to the guidelines.
  • I/We Author(s ) have niether given nor will give this manuscript elsewhere for publishing after submitting in STM Journal(s).
  • I/ We have read the original version of the manuscript and am/ are responsible for the thought contents embodied in it. The work dealt in the manuscript is my/ our own, and my/ our individual contribution to this work is significant enough to qualify for authorship.
  •  I/We also agree to the authorship of the article in the following order:

Author’s name                                    

 

1. ________________

2. ________________

3. ________________

4. ________________

 

We  Author(s) tick this box and would request you to consider it as our signature as we agree to the terms of this Copyright Notice, which will apply to this submission if and when it is published by this journal.