Chemical Constituents and Tyrosinase Inhibitory Activity of Aqueous Fraction of the Leaves of Morus alba L. from Vietnam

Abstract

The mulberry has been widely cultivated to feed silkworms. The leaves of mulberry have been used in traditional medicine as an analgesic, antitussive, cathartic, diuretic. In this study we have isolated three compounds (LC1-3) by chromatographic methods from the Mulberry leaves (Morus alba L.) collected in Thai Nguyen province, Vietnam. These compounds were identified as: Kaempferol-3,7-di-O-α-L-rhamnopyranoside (LC1), 7,4^’-dihydroxy-5,3^’-dimethoxyflavone (LC2), (S)-5,5^’,7-trihydroxy-2^’,4^’-dimethoxy-6-methylflavanone (LC3). Their structures were elucidated by spectroscopic methods, including MS and NMR. Compound LC3 was isolated from Mulberry leaves for the first time. These compounds were evaluated the tyrosinase inhibitory activity in vitro. Our data showed that compound LC3 has potential tyrosinase inhibitory effects with IC₅₀ values of 15.48 ± 2.96 µg/mL.

Keyword: Morus alba L., tyrosinase inhibitors, Kaempferol-3,7-di-O-α-L-rhamnopyranoside, 7,4^’-dihydroxy-5,3^’-dimethoxyflavone,(S)-5,5^’,7-trihydroxy-2^’,4^’-dimethoxy-6-methylflavanone.

Cite this Article

Loi Vu Duc, Tung Bui Thanh, Huong Le Thi Thu et al. Chemical Constituents and Tyrosinase Inhibitory Activity of Aqueous Fraction of the Leaves of Morus alba L. from Vietnam. Research & Reviews: A Journal of Drug Formulation, Development and Production. 2017; 4(3): 12–17p.