Isolation, Characterization of Rhinacanthus Nasutus L. Kurz and Its Biological Evaluation

Abstract

The present paper deals with isolation, characterization and evaluation of anti-inflammatory, analgesic and anti-bacterial activities of n-hexane extract of Rhinacanthus nasutus L. Kurz. Extraction was carried out with soxhlet apparatus using solvents of increasing polarity. The anti-inflammatory activity was done by Carrageenan-Induced Paw Edema in rats. Analgesic activity was done by hot plate method and anti-bacterial activity by plate hole diffusion method. n-Hexane extract exhibited significant change in anti-inflammatory, analgesic activities when compared with the standard drug and control. Antibacterial screening of n-hexane extract was 100% active in concentration of 1000 µg/ml, 93% active in concentration of 500 µg/ml, and 87.5% active in concentration of 250 µg/ml.

 Keywords: rhinacanthus nasuta (L) kurz, phytochemical screening, anti-inflammatory, 

analgesic, anti-bacterial.