Antibacterial Activities of Crude Extract from Infected Cocoa Against Abscess Forming Bacteria

Abstract

In this study, the antibacterial activity of crude phytoalexin extracted from matured cocoa pod against abscess forming bacteria was investigated. The crude phytolexin was extracted using redistilled dichloromethane after five days of post infection. Different concentrations (50, 100, 150 and 200 mg/ml) of the extract were tested against abscess forming bacteria namely; Escherichia coli, Staphylococcus aureus, Klebsiella Pneumoniae, Pseudomonas aeruginosa and Enterococcus faecium using paper disc and agar diffusion methods. The result of paper disc method showed that E. coli had a zone of inhibition that varied from 3.00 (50 mg/ml) to 8.00 mm (200 mg/ml), S. aureus 2.00 (50 mg/ml) to 6.00 mm (200 mg/ml), K. pneumonia 2.0 (50 mm) to 9 mm (200 mg/ml), P. aeruginosa 2 mm (50 mg/ml) to 6.00 mm (200 mg/ml) and E. faecium 3.00 (50 mg/ml) to 9.00 mm (200 mg/ml) while the control showed no zone of inhibition. Also, the result of agar diffusion method showed that E. coli had zone of inhibition that varied 6 (50 mg/ml) to 14 mm (200 mg/ml), S. aureus 5.00 (50 mg/ml) to 15.00 mm (200 mg/ml), K. pneumoniae 4.00 (50 mm) to 15 mm (200 mg/ml), P. aeruginosa 6 (50 mg/ml) to 16.00 mm (200 mg/ml) and E. faecium 3.00 (50 mg/ml) to 9.00 mm (200 mg/ml). From the results of this study, it is concluded that the extract from infected cocoa pods can be employed in the production of antibiotics to combat abscess forming bacteria.

Keywords: Abscess, cocoa, extract, phytoalexin, antibacterial, inhibition

Cite this Article

Fagbohun ED, Ibrahim TA. Antibacterial activities of crude extract from infected Cocoa against abscess forming bacteria. Research & Reviews: A Journal of Pharmacognosy. 2015; 2(3): 21–27p.