Wound Healing Effect of Methanolic Extract of Barringtonia acutangula Fruits in Streptozotocin Induced Diabetic Rats

Abstract

The present study is undertaken to evaluate wound healing effect of methanolic extract of Barringtonia acutangula fruits (MEBA) in streptozotocin induced diabetic rats. Diabetes is induced by administration of streptozotocin 45 mg/kg i.p. Both excision and incision models were used in rats. In the excision wound model, the wound area of each animal was measured on days 0, 2, 4, 6, 8, 10, 12 and 14 after inflicting the wound. Wound contraction (WC) was calculated as a percentage change in the initial wound size. The histopathology study of the skin (newly formed on the wounds) was carried out on 14th post wounding day. Hydroxyproline content was also measured. In the incision wound model, the tensile strength was measured by using tensiometer on 14th post wounding day. MEBA at 20% w/w showed significant wound healing activity as compared to control. The percentage of wound contraction on 14th day by MEBA at 5, 10 and 20% w/w were found to be 90.50, 93.49 and 96.32% respectively. The histopathology revealed an increased number of fibroblast, increased collagen tissue and complete epithelialization in case of MEBA ointment 20% w/w. The tensile strength and hydroxyproline content were found to be significantly increased. Methanolic extract of Barringtonia acutangula fruit (ointment, 20% w/w) showed significant wound healing activity in both excised wound model and incision wound model.

Keywords: Barringtonia, diabetes, streptozotocin, wound healing

Cite this Article

Mohanty A, Das C, Prusty SK, et al. Wound Healing Effect of Methanolic Extract of Barringtonia acutangula Fruits in Streptozotocin Induced Diabetic Rats. Research & Reviews: A Journal of Pharmacognosy. 2016; 3(1): 24–29p.