New RP-HPLC Method Development and Validation for Simultanious Estimation and Forced Degradation Studies of Gallic acid and Curcumin in Solid Dosage Form

Authors

  • S K Godasu Research scholar, Department of Pharmacy, Mewar University, Chittorgarh, Rajasthan, India.
  • S. A. Sreenivas Supervisor, Department of Pharmacy, Mewar University, Chittorgarh, Rajasthan, India.

Keywords:

Gallic acid, curcumin, RP?HPLC, estimation, Chromatographic separations

Abstract

Objective: A New method was established for simultaneous estimation of Gallic acid and curcumin by RP-HPLC method. Methods: Chromatographic separations were performed on an Inspire (4.6 x 150mm, 5m) column with a mobile phase composition of 0.1 percent OPA buffer and Acetonitrile (30:70) delivered at a flow rate of 1 ml/min, and detection was performed on a waters HPLC auto sampler, separation module 2695 with PDA detector 2996 at wavelength 260 nm. Results: The retention time for Gallic acid and curcumin were 2.119 and 2.730 minute respectively. The linearity correlation coefficients for Gallic acid and curcumin were 0.999 and the concentration ranges were 500-2500 g/ml and 5-25 g/ml, respectively. For accuracy the total recovery was found to be 100.58% and 100.54% for Gallic acid and curcumin. LOD and LOQ for gallic acid 3.05 and 10.07. LOD and LOQ for Curcumin 2.28 and 9.98. Conclusion: The study's findings revealed that the suggested RPHPLC method is a simple, accurate, precise, rugged, robust, rapid, and repeatable method for routinely estimating Gallic acid and curcumin in pharmaceutical dose form.

References

Gallic acid properties available from URL: Chemicalland 21. Gallic Acid. Online available at: http://www.chemicalland21.com/specialtychem/finechem/GALLIC%20ACID.htm.

Abbasi S, Daneshfar A, Farmany A, et al. Quantification of subnanomolar levels of gallic acid by adsorptive stripping voltametry, International Jornal of Electrochemical Science. 6, 2011: 4843-4852.

Turmeric-curcumin. WHAT ARE TURMERIC AND CURCUMIN? Online availlable at: https://turmeric-curcumin.com/

Lutomski J, Kedzia B, and Debska W. Effect of an alcohol extract and of active ingredients from Curcuma longa on bacteria and fungi. Planta Medica. 1974 Aug;26(1):9-19.

Bhavani S and Sreenivasa M. Effect of turmeric (Curcuma longa) fractions on the growth of some intestinal and pathogenic bacteria in vitro. Indian Journal Experimental Biology. 1979 Dec;17(12):1363-6.

Sonawane D, Nirmal S, Patil N, et al, Development and validation of HPTLC method to detect curcumin and gallic acid in polyherbal formulation, Journal of Liquid Chromatography & Related Technologies 2011;34(20): 2664–2673.

Rubesh Kumar S, Ram Kishan J, Duganath N, et al. Simultaneous spectrophotometric estimation of curcuminoids and gallic acid in bulk drug and ayurvedic polyherbal tablet dosage form, International Journal of Pharmaceutical Quality Assurance. 2010:2(1): 56-59.

Kilambi P and Dave H. Simultaneous determination of curcumin and berberine in their pure form and from the combined extracts of curcuma longa and berberisaristata, International Journal of Applied Science and Engineering. 2010:8(1):19-26.

Soni H, Patel S, Nayak G, et al. Qualitative and quantitative profile of curcumin from ethanolic extract of curcuma longa, International Research Journal of Pharmacy. 2011:2(4):180-184.

Sharma K, Gupta M and Agrawal S. Development and validation of UV spectrophotometric method for the estimation of curcumin in bulk drug and pharmaceutical dosage forms, International Journal of Drug Development & Research. 2012:4(2):375-380.

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Published

2022-05-23

Issue

Vol. 13 No. 1 (2022)

Section

Research Article

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