Interaction of Cardiotoxin with Calmodulin and its EF Hand Peptides
DOI:
https://doi.org/10.37591/tdd.v4i3.63Abstract
Calmodulin is a ubiquitous protein having EF hand peptide(helix-loop-helix sstructural domain), which is found in all the eukaryotic cells. Calmodulin functions as a multipurpose intracellular receptor, mediating much calcium regulated processes and undergoes conformational change upon binding to calcium. These activated calmodul in then bind to a number of different target proteins, including a family of calcium/calmodul in-dependent protein kinases which then phosphorylatedserine’s or threnodies on other proteins. Cardiotoxin, snake toxins are known to act through the various cellular pathways. Many target proteins are known to bind calmodulin and exert their function therefore, this study has been designed in such a way to study the interaction of cardiotoxin three with calmodulin. Calmodulin was isolated from bovine brain, tissue was subjected to homogenisation followed by hydrophobic interaction chromatography. Stains—all, a carbocyanine dye was used in this study to record circular dichroism spectra. There was change in circular dichroism spectra when cardiotoxin was added to calmodulin-stains all complex. This decrease in J band on concomitant addition of cardiotoxin to calmodulin–satins all complex which show that there is interaction between Calmodulin and cardiotoxin. Cardiotoxin therefore by binding to calmodulin effect various biological process this needs to be studied in future.
Keywords: Calmodulin, cardiotoxin, calcium, Circular Dichroism
Cite this Article
Priyanka Verma. Interaction of Cardiotoxin with Calmodulin and its EF Hand Peptides. Trends in Drug Delivery. 2017; 4(3): 9–13p.
Downloads
Published
Issue
Section
License
Declaration and Copyright Transfer Form
(to be completed by authors)
I/ We, the undersigned author(s) of the submitted manuscript, hereby declare, that the above manuscript which is submitted for publication in the STM Journals(s), is not published already in part or whole (except in the form of abstract) in any journal or magazine for private or public circulation, and, is not under consideration of publication elsewhere.
- I/We will not withdraw the manuscript after 1 week of submission as I have read the Author Guidelines and will adhere to the guidelines.
- I/We Author(s ) have niether given nor will give this manuscript elsewhere for publishing after submitting in STM Journal(s).
- I/ We have read the original version of the manuscript and am/ are responsible for the thought contents embodied in it. The work dealt in the manuscript is my/ our own, and my/ our individual contribution to this work is significant enough to qualify for authorship.
- I/We also agree to the authorship of the article in the following order:
Author’s name
1. ________________
2. ________________
3. ________________
4. ________________
| We Author(s) tick this box and would request you to consider it as our signature as we agree to the terms of this Copyright Notice, which will apply to this submission if and when it is published by this journal. |
